Here are some of the empirical studies that I read regarding the use of 3D virtual environments in education :
Clark, S., & Maher, M. L. (2005). Learning and Designing in a Virtual Place: Investigating the Role of Place in a Virtual Design Studio, Proceedings of Ecaade 2005,
Clark, S., & Maher, M. L. (September 2003). The Effects of a Sense of Place on the Learning Experience in a 3D Virtual World. In Cook, J. and McConnell, D. (Eds). Communities of Practice. Research Proceedings of the 10th Association for Learning Technologies Conference (ALT-C2003), pp 82-101
Dickey, M. D. ( 2005). Three-dimensional virtual worlds and distance learning: two case studies of Active Worlds as a medium for distance education, British Journal of Educational Technology. 36, 439-451.
Dickey, M. D. (2003). Teaching in 3D: Pedagogical Affordances and Constraints of 3D Virtual Worlds for Synchronous Distance Learning. Distance Education, 24, 105-121.
Mikropoulos, T. A. (2001). Brain activity on navigation in virtual environments. Journal of Educational Computing Research, 24 (1), 1-12.
Di Blas, N., Paolini, P., & Poggi, C. (2004). Learning by Playing. An Edutainment 3D Environment for Schools. In Proceedings of ED-MEDIA'04---World conference on educational multimedia and hypermedia. Lugano: AACE.
Delwiche, A. (2006). Massively multiplayer games (MMOs) in the new media classroom. Educational Technology and society, 9, 160-172.
Mumtaz, S. (2001). Children’s enjoyment and perception of computer use in the home and the school. Computers & Education, 36, 347–362.
Virvou, M., Katsionis, G., & Manos, K. (2005). Combining Software Games with Education: Evaluation of its Educational Effectiveness. Educational Technology & Society, 8, 54-65.
Elliott, J., Adams, L., Bruckman, A. No magic bullet: 3D video games in education. In Proceedings of International Conference of the Learning Sciences. (